产品货号：
GS2147
中文名称：
Bsp1720I内切酶
英文名称：
Bsp1720I
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：Bsp1720I内切酶，Bsp1720I限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GC↑TNAGC |
| 来源 | Bacillus species 1720 |
| Assayed on | Lambda DNA |
| Optimal buffer | G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme about 80%of DNA fragment can be ligated.Of these about 95%can be recut |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃ |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BlpI,Bpu1102I,CelII,EspI |
相关搜索：Bsp1720I内切酶，Bsp1720I限制性内切酶